Improving adventitious shoot regeneration from cultured leaf explants of Petunia hybrida using thidiazuron
نویسنده
چکیده
The effect of various concentrations of thidiazuron (TDZ) with or without 2.7 μM of α-naphthalene acetic acid (NAA) on adventitious shoot formation of two Petunia hybrida cultivars was studied. Seeds from ‘Daddy Blue’ and ‘Dreams White’ cultivars were germinated in vitro. Expanded leaves from both seedlings and regenerated shoots were taken as explants. Explants were placed on MS media supplied with different concentrations and combinations of the growth regulators. Cultures were incubated under dark conditions for three weeks and then moved under light condition. The ability to form shoots varied depending on the growth regulator combination. The highest shoot percentage (87.5 to 90%) with both explant sources was obtained when TDZ was used at levels between 0.5 and 8 μM in both cultivars. Regeneration was reduced significantly when TDZ was combined with 2.7 μM NAA. A similar trend of average number of shoots produced was observed.
منابع مشابه
بهبود اندامزایی شاخساره نابهجا و تشکیل پینه از ریزنمونههای برگ اطلسی دورگه (.Petunia hybrida L) با استفاده از بنزیل آدنین (BA) و نفتالن استیک اسید (NAA)
Petunia (Petunia hybrida) is an ornamental plant species of high economic potential. One of the appropriate methods for propagation and breeding of petunia is tissue culture. This experiment was conducted to investigate the effects of different concentrations of benzyl adenine (BA) either alone or in combination with different concentrations of naphthalene acetic acid (NAA) on the regeneration ...
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The DNA methylase inhibitors, 5-azacytidine and 5-aza-2'-deoxycytidine inhibited adventitious shoot induction in Petunia leaf cultures. Cytosine methylation status at CCGG sites in shoot- and callus-inducing culture treatments was analysed by coupled restriction enzyme digestion (HpaII or MspI) and random amplification. Two differentially methylated genomic DNA bands from the PCR products were ...
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